Science / Technology - Colloquium
Monday, April 28, 2008
4:00 PM-5:00 PM
Olin Hall
107
We have developed microfluidic tools for studying populations of single
cells. We encapsulate individual cells using polydimethylsiloxane
(PDMS) devices. Cells are trapped either in chambers as wide as a
single cell and long enough to contain many generations of cells, or in
15 micron droplets of a water-in-oil emulsion generated in PDMS
microfluidic devices. In the chambers, cells are maintained under
constant flow conditions, and divide for many generations. All progeny
deriving from the single cell are contained in the chamber, facilitating
studies of gene expression, stress response, and DNA repair as a
function of cell genealogy and replicative age. Drops of a water-in-oil
emulsion also function to isolate single cells over the experimental
timecourse. We have developed a device to immobilize drops, allowing us
to track single cell growth, and to quantify levels of secreted enzymes
that rapidly attain high concentrations due to the small drop volume.
Cost: FREE
Sponsored by: WPI Physics Department, Dr. Stephan Koehler
Suggested Audiences: College
E-mail:
sak@wpi.edu
Phone: 508-831-5090
Last Modified: April 15, 2008 at 3:19 PM
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