Physics Department Colloquium, "Genomic Applications of Droplet Microfluidics," by Darren R. Link, Co-founder and Vice President of Research & Development, RainDance Technologies, Inc. Lexington, MA
Science / Technology - Colloquium
Monday, April 22, 2013
4:00 PM-5:00 PM
The need for detecting, quantifying and monitoring genetic changes in cancers is driving the development of new tools for human genetic analysis. At RainDance Technologies we are developing microfluidic tools to meet these analytical demands. The first of these tools provides a method for uniformly enriching thousands of different target portions of a patient's genome prior to DNA sequencing analysis, by preparing millions of individual PCR reactions in microfluidic droplets. This enrichment enables an oncologist to identify and quantify low prevalence mutations in heterogeneous tumor samples. A second tool exploits the small volume and confinement of picoliter-sized droplets to set up and fluorescently count up to 10 million single-molecule digital PCR reactions, precisely quantifying the presence of low level mutations in a high background of wild-type DNA. Microfluidic-enabled assays like these promise to allow 'liquid biopsies' for detecting cancer earlier and monitoring drug effectiveness without using invasive procedures.
Refreshments will be served in Olin Hall 118 at 3:30 P.M.
Sponsored by: WPI Physics Department
Suggested Audiences: College
Last Modified: April 16, 2013 at 10:30 AM